Faculty : Departmental- Primary

Noriyuki Kasahara, M.D., Ph.D.

Current projects span three different areas:

1) Translational development of tumor-selective replicating retroviral vectors (RRV) for gene therapy of cancer, currently being evaluated in first-in-human Phase I/II multi-center clinical trials for patients with recurrent high-grade glioma;

2) Genetic engineering of mesenchymal stem cells and T cells to serve as tumor-homing vector producer cells, toward development of combined strategies for cancer gene therapy and immunotherapy;

3) Genetic engineering of stem cells to reduce graft rejection by modulating histocompatibility, and to enable selective in vivo expansion of engrafted cells by conferring chemo-resistance.

Professional Affiliations

University of Miami Sylvester Comprehensive Cancer Center

Select Publications

2014

Takahashi, M., Valdes, G., Hiraoka, K., Inagaki, I., Kamijima, S., Micewicz, E., Gruber, H. E., Robbins, J. M., Jolly, D. J., McBride, W. H., Iwamoto, K. S., Kasahara, N. (2014) Radio-sensitization of gliomas by intracellular generation of 5-fluorouracil potentiates prodrug activator gene therapy with a retroviral replicating vector. Cancer Gene Therapy, in press.

Lin, A. H., Timberlake, N., Logg, C. R., Liu, Y., Kamijima, S., Diago, O., Wong, K., Gammon, D. K., Ostertag, D., Hacke, K., Yang, E. C., Gruber, H., Kasahara, N., Jolly, D. J. (2014) MicroRNA 142-3p Attenuates Spread of Replicating Retroviral Vector in Hematopoietic Lineage-Derived Cells While Maintaining an Antiviral Immune Response. Human Gene Therapy, Jun 18. [Epub ahead of print]

Mintz, P. J., Huang, K. W., Reebye, V., Nteliopoulos, G., Lai, H. S., Saetrom, P., Kasahara, N., Jensen, S., Madhava, P., Gordon, M. Y., Marley, S. B., Behan, R., Spalding, D. R., Haoudi, A., Emara, M. M., Nicholls, J., Rossi, J. J., Habib, N. A. (2014) Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Molecular Therapy, 22: 149-159.

2013

Kubo, S., Takagi-Kimura, M., Logg, C. R., Kasahara, N. (2013) Highly efficient tumor transduction and antitumor efficacy in experimental human malignant mesothelioma using replicating gibbon ape leukemia virus. Cancer Gene Therapy, 20: 671-677.

Hacke, K., Treger, J. A., Bogan, B. T., Schiestl, R. H., Kasahara, N. (2013) Genetic modification of mouse bone marrow by lentiviral vector-mediated delivery of hypoxanthine-guanine phosphoribosyltransferase short hairpin RNA confers chemoprotection against 6-thioguanine cytotoxicity. Transplantation Proceedings, 45: 2040-2044.

Takagi-Kimura, M., Yamano, T., Tamamoto, A., Okamura, N., Okamura, H., Hashimoto-Tamaoki, T., Tagawa, M., Kasahara, N., Kubo, S. (2013) Enhanced antitumor efficacy of fiber-modified, midkine promoter-regulated oncolytic adenovirus in human malignant mesothelioma. Cancer Science, 104: 1433-1439.

Pham, L. K., Liang, M., Adisetiyo, H., Liao, C. P., Cohen, M. B., Tahara, S. M., Frenkel, B., Kasahara, N., Roy-Burman, P. (2013) Contextual effect of repression of bone morphogenetic protein activity in prostate cancer. Endocrine-Related Cancer, 20: 861-874.

Hickey, M. J., Malone, C. C., Erickson, K. E., Lin, A., Soto, H., Ha, E. T., Kamijima, S., Inagaki, A., Takahashi, M., Kato, Y., Kasahara, N., Mueller, B., Kruse, C. A. (2013) Combined Alloreactive CTL Cellular Therapy with Suicide Prodrug Activator Gene Therapy in a Model of Breast Cancer Metastatic to the Brain. Clinical Cancer Research, 19: 4137-4148.

2012

Logg, C. R., Robbins, J. M., Jolly, D. J., Gruber, H. E., Kasahara, N. (2012) Retroviral Replicating Vectors in Cancer. Methods in Enzymology, 507: 199-228.

Hacke, K., Szakmary, A., Cuddihy, A. R., Rozengurt, N., Lemp, N. A., Aubrecht, J., Lawson, G. W., Rao, N. P., Crooks, G. M., Schiestl, R. H., Kasahara, N. (2012) Combined preconditioning and in vivo chemoselection with 6-thioguanine alone achieves highly efficient reconstitution of normal hematopoiesis with HPRT-deficient bone marrow. Experimental Hematology, 40 (1): 3-13. e3.

Voutila, J., Sætrom, P., Mintz, P., Sun, G., Alluin, J., Rossi, J. J., Habib, N.A., Kasahara, N. (2012) Gene Expression Profile Changes After Short-activating RNA-mediated Induction of Endogenous Pluripotency Factors in Human Mesenchymal Stem Cells. Molecular Therapy – Nucleic Acids, 1: e35

Perez, O., Logg, C. R., Hiraoka, K., Diago, O., Burnett, R., Inagaki, A., Jolson, D., Amundsen, K., Buckley, T., Lohse, D., Lin, A., Burrascano, C., Ibanez, C., Kasahara, N., Gruber, H., Jolly, D. J. (2012) Design and Selection of Toca 511 for Clinical Use: Modified Retroviral Replicating Vector with Improved Stability and Gene Expression. Molecular Therapy, 20: 1689-1698.